吳忠義
吳忠義博士，研究員。1969年(nián)生于福建德化，1988年(nián)7月至1998年(nián)6月就讀于中國農業大學(xué)并獲得學(xué)士、碩士和(hé)博士學(xué)位。2002年(nián)入選北京市科技新星；首都師範大學(xué)碩士生導師；北京市農林科學(xué)院博士後工作站博士後合作導師。 1998年(nián)7月至今在北京市農林科學(xué)院生物技術研究所工作。 1999年(nián)3月至2002年(nián)3月在美國馬裏蘭大學(xué)細胞與分子(zǐ)遺傳系作博士後。
研究方向
長(cháng)期從事植物逆境分子(zǐ)生物學(xué)和(hé)生物技術育種方面工作，目前主要從事玉米分子(zǐ)育種工作，緻力于建立和(hé)完善不依賴于基因型的(de)玉米骨幹自(zì)交系的(de)高(gāo)效DNA導入技術，建立和(hé)完善玉米骨幹自(zì)交系的(de)基因編輯技術體系，通過促進玉米根系生長(cháng)培育抗旱玉米新種質材料。 熟悉學(xué)科領域：水分與礦質元素代謝與運輸，作物抗逆生理(lǐ)，基因組編輯，植物激素與生長(cháng)調節物質，作物分子(zǐ)育種，逆境信号轉導等。
科研成果
近期主要主持參加項目
| [1] | 基于納米磁珠介導的(de)花粉轉染法開發新型高(gāo)效玉米轉化體系（主持院創新能力儲備性項目2019-2022，90萬元） |
| [2] | 花粉管導入的(de)玉米骨幹自(zì)交系基因組編輯技術研究與應用（主持北京市科技計劃項目2017-2020，210萬元） |
| [3] | 基因組編輯技術在玉米林草(cǎo)花的(de)研究應用（參加院創新能力建設2018-2019，50萬元） |
| [4] | 基于組學(xué)的(de)玉米維管束形成機理(lǐ)解析（參加院創新能力建設2017-2019，84萬元） |
| [5] | 重要農作物轉基因新技術研究子(zǐ)課題（參加院創新能力建設2017-2019，90萬元） |
| [6] | 重要作物分子(zǐ)育種平台關鍵技術研發（參加院創新能力建設2016-2018，75萬元） |
| [7] | 促進根系發育來培育和(hé)脅迫響應的(de)多價轉基因抗旱玉米新品種培育(國家轉基因專項重點課題2012ZX08003-003B, 2014-2016, 90萬元，子(zǐ)課題主持) |
| [8] | 作物分子(zǐ)育種關鍵技術與新種質創制（參加院創新能力專項子(zǐ)課題105萬元. 2014-2016） |
主要代表論文及授權專利
| [1] | Chun Zhang, Ruijia Yang, Dengyu Zhen, Xianglong Li, Zhongbao Zhang*, Legong Li* and Zhongyi Wu*. ZmTIFY16, a novel maize TIFY transcription factor gene, promotes root growth and development and enhances drought and salt tolerance in Arabidopsis and Zea mays. Plant Growth Regulation, 2023 https://doi.org/10.1007/s10725-022-00946-2 |
| [2] | Zuoping Wang, Zhongbao Zhang, Dengyu Zheng, Tongtong Zhang, Xianglong Li, Chun Zhang, Rong Yu, Jianhua Wei* and Zhongyi Wu*. Efficient and genotype independent maize transformation using pollen transfected by DNA coated magnetic nanoparticles. J. Integr. Plant Biol. 2022, 64: 1145–1156. https://doi.org/10.1111/jipb.13263 |
| [3] | Zhongbao Zhang, Xiaoqin Tan, Xiaorong Sun, Jianhua Wei*, Qing Xiao Li* and Zhongyi Wu*. Isoorientin affects markers of Alzheimer’s disease via effects on the oral and gut microbiota in APP/PS1 mice. Journal of Nutrition, 2022, 152：140-152. |
| [4] | Chun Zhang, Xianglong Li, Zuoping Wang, Zhongbao Zhang* and Zhongyi Wu*. Identifying key regulatory genes of maize root growth and development by RNA sequencing. Genomics, 2020, 112: 5157–5169. |
| [5] | Zhongbao Zhang, Longfei Yin, Xianglong Li, Chun Zhang, Cai Liu and Zhongyi Wu*. Analyses of the Complete Genome Sequence of the Strain Bacillus pumilus ZB201701 Isolated from Rhizosphere Soil of Maize under Drought and Salt Stress. Microbes Environ.2019,34(3): 310-315. |
| [6] | Zhongbao Zhang, Zhibin Liang, Longfei Yin, Qingxiao Li* and Zhongyi Wu*. Distribution of four bioactive flavonoids in maize tissues of five varieties and correlation with expression of the biosynthetic genes. Journal of Agricultural and Food Chemistry, 2018, 66 (40): 10431-10437. （封面論文） |
| [7] | Zhongbao Zhang, Longfei Yin, Xianglong Li, Chun Zhang, Cai Liu and Zhongyi Wu*. The complete genome sequence of Bacillus halotoleransZB201702 isolated from a drought- and salt-stressed rhizosphere soil. Microbial Pathogenesis, 2018, 123: 246–249. |
| [8] | Zhongbao Zhang, Xianglong Li, Chun Zhang and Zhongyi Wu*. Isolation, structural analysis, and expression characteristics of the maize nuclear factor Y gene families. Biochemical and Biophysical Research Communications. 2016, 478: 752-758. |
| [9] | Zhongbao Zhang, Xianglong Li, Rong Yu, Meng Han and Zhongyi Wu*. Isolation, structural analysis, and expression characteristics of the maize TIFY gene family. Mol Genet Genomics. 2015, 290: 1845-1858. |
| [10] | Huawen Zou, Xiaohan Wang, Conglin Huang, Jinsong Chen, Xiuhai Zhang, Chang Luo, Rong Yu and Zhongyi Wu*. Stress-inducible expression of a gene encoding C-repeat binding factor 4 (CBF4) from Arabidopsis improved performance of transgenic maize under drought condition. Plant Omics Journal. 2014, 7: 94-101. |
| [11] | Xiaohan Wang, Binlin Liu, Conglin Huang, Xiuhai Zhang, Chang Luo, Xi Cheng, Rong Yu and Zhongyi Wu*. Over expression of Zmda1-1 gene increases seed mass of corn. African Journal of Biotechnology. 2012, 11(69): 13387-13395 |
| [12] | Jinsong Chen, Conglin Huang, Xiuhai Zhang, Rong Yu and Zhongyi Wu*. Detection of herbicide-resistant maize by using Loop-Mediated Isothermal Amplification of the pat selectable marker gene. African Journal of Biotechnology, 2011, 10(75): 17055-17061 |
| [13] | Yongmei Zhang, Zhongyi Wu and Rong Yu. Rearrangements of microtubule cytoskeleton in stomatal closure of Arabidopsis induced by nitric oxide. 2008, Chinese Science Bulletin, 2008, 53(6):848-852. |
| [14] | Huawen Zou, Zhongyi Wu, XiuhaiZhang, Qin Yang, Wensuo Jia and Conglin Huang. Gene expression analysis of ZmPti1, encoding a maize Pti like kinase, suggest a role in stress signalling. Plant Science, 2006, 171: 99-105 |
| [15] | Yuhua Wang, Zhongyi Wu, Xiuhai Zhang, Guoqiang Chen, Qiong Wu, Conglin Huang and Qing Yang. Synthesis of medium-chain-length-polyhydroxyalkanoates in tobacco via chloroplast genetic engineering. Chinese Science Bulletin, 2005, 50: 1113-1120 |
| [16] | Zhongyi Wu, Feng Liang, Bimei Hong, Jeff C. Young, Michael R. Sussman, Jeffrey F. Harper and Heven Sze. An ER-bound Ca2+/Mn2+pump, ECA1, supports plant growth and confers tolerance to Mn2+stress. Plant Physiol. 2002, 130：128-137. |
| [17] | 吳忠義，黃叢林，張秀海等，授權專利名稱“一(yī)種玉米ZmPti1基因啓動子(zǐ)及其應用”，中國，2011，專利證書号：ZL200810227596.3。 |
| [18] | 吳忠義，黃叢林，張秀海等，授權專利名稱“一(yī)種利用LAMP法快速檢測轉基因玉米的(de)方法”，中國，2012，專利證書号：ZL2010120766.5。 |
| [19] | 吳忠義，黃叢林，張秀海等，授權專利名稱“一(yī)種拟南芥根特異性表達基因ARSK1啓動子(zǐ)的(de)克隆方法”，中國，2013，專利證書号：ZL 201110359401.2 |
| [20] | 李向龍，吳忠義，張曉東等，授權專利名稱“一(yī)種提高(gāo)玉米授粉效率的(de)授粉方法”，中國，2016，專利号：ZL201510416431.09401.2 |
| [21] | 王作平，吳忠義，張中保，李向龍，張春。授權專利名稱“一(yī)種不依賴于玉米基因型的(de)DNA導入方法” 專利号：ZL201910623296.5 |
| [22] | 張中保，吳忠義，鄭登俞，王作平，張春，李向龍。授權專利名稱“一(yī)種改良的(de)玉米花粉磁轉染方法”專利号：ZL202111418727.8 |
| [23] | 吳忠義，張中保，王作平，鄭登俞，張春，李向龍。授權專利名稱“一(yī)種制備轉基因玉米花粉的(de)方法及其使用的(de)試劑盒” 專利号：ZL202210532986.1 |
